Caught In The Act: Butterfly Mate Preference Shows How One Species Can Become Two

Breaking up may actually not be hard to do, say scientists who've found a population of tropical butterflies that may be on its way to a split into two distinct species.

The cause of this particular break-up? A shift in wing color and mate preference.

In a paper published this week in the journal Science, the researchers describe the relationship between diverging color patterns in Heliconius butterflies and the long-term divergence of populations into new and distinct species.

"Our paper provides a unique glimpse into the earliest stage of ecological speciation, where natural selection to fit the environment causes the same trait in the same population to be pushed in two different directions," says Marcus Kronforst, a Bauer Fellow in the Center for Systems Biology at Harvard University who received his doctor's degree at The University of Texas at Austin. "If this trait is also involved in reproduction, this process can have a side effect of causing the divergent subpopulations to no longer interbreed. This appears to be the process that is just beginning among Heliconius butterflies in Ecuador."

Heliconius butterflies display incredible color pattern variation across Central and South America, with closely related species usually sporting different colors. In Costa Rica, for example, the two most closely related species differ in color: One species is white and the other is yellow. In addition, both species display a marked preference to mate with butter-flies of the same color.

The Ecuadorian population examined by Kronforst and his colleagues shows the same white and yellow variation found in Costa Rica but has not yet reached a level of strong reproductive isolation. The entire population lives in close proximity and individuals of both colors come in contact with -- and mate with -- each other.

But, by studying the Ecuadorian population in captivity, the scientists found the two colors do not mate randomly. Despite the genetic similarity between the groups -- white and yellow varieties differ only at the color-determining gene -- yellow Ecuadorian individuals show a preference for those of the same color. White male butterflies, most of which are heterozygous at the gene that controls color, show no color preference.

"This subtle difference in mate preference between the color forms in Ecuador may be the first step in a process that could eventually result in two species, as we see in Costa Rica," says Kronforst, who began studies of Heliconius color pattern and behavioral genetics in the laboratory of Professor Lawrence Gilbert at The University of Texas at Austin.

Previous studies of species formation have focused on the characteristics of well-differentiated species, and the health and viability of their hybrids in particular, in an effort to identify how the species may have emerged and how they stay distinct.

Heliconius provides a model for a different kind of study. The researchers considered species emergence from the opposite end, studying populations that have yet to diverge into separate species in order to identify the role of mate choice in the potential emergence of new species.

Having identified color-based mate preference in Heliconius, the researchers used a battery of genetic markers to compare the genomes of the white and yellow varieties, showing that they are genetically identical except for their different colors and preferences.

Their work suggests that the genes for color and preference are very close to one another in the genome; the two traits could even be caused by the same gene. Their next step is to identify the gene (or genes) responsible for the differences in color and mate preference.

"If we can identify this gene or genes, we can say conclusively how they influence both color and mate choice," says Kronforst. "Subsequent work could elucidate exactly how changes in individual genes can, over long periods of time, lead to novel species."

"This study shows the great potential of the genus Heliconius as a model system for integrating genetics, development, behavior, ecology and evolution," says Gilbert, professor in the Section of Integrative Biology. "It is the culmination of diverse contributions of the co-authors involving insectary, field and laboratory research over more than a decade."

Co-authors on the Science paper with Kronforst are Nicola L. Chamberlain and Ryan I. Hill, both of Harvard; Durrell D. Kapan of the University of Hawaii; and Lawrence E. Gilbert of The University of Texas at Austin. Their work was funded by the National Science Foundation and the National Institutes of Health.

Biological Clocks Discovery Overturns Long-held Theory

University of Michigan mathematicians and their British colleagues say they have identified the signal that the brain sends to the rest of the body to control biological rhythms, a finding that overturns a long-held theory about our internal clock.

Understanding how the human biological clock works is an essential step toward correcting sleep problems like insomnia and jet lag. New insights about the body's central pacemaker might also, someday, advance efforts to treat diseases influenced by the internal clock, including cancer, Alzheimer's disease and mood disorders, said University of Michigan mathematician Daniel Forger.

"Knowing what the signal is will help us learn how to adjust it, in order to help people," said Forger, an associate professor of mathematics and a member of the U-M's Center for Computational Medicine and Bioinformatics. "We have cracked the code, and the information could have a tremendous impact on all sorts of diseases that are affected by the clock."

The body's main time-keeper resides in a region of the central brain called the suprachiasmatic nuclei, or SCN. For decades, researchers have believed that it is the rate at which SCN cells fire electrical pulses---fast during the day and slow at night---that controls time-keeping throughout the body.

Imagine a metronome in the brain that ticks quickly throughout the day, then slows its pace at night. The rest of the body hears the ticking and adjusts its daily rhythms, also known as circadian rhythms, accordingly.

That's the idea that has prevailed for more than two decades. But new evidence compiled by Forger and his colleagues shows that "the old model is, frankly, wrong," Forger said.

The true signaling mechanism is very different: The timing signal sent from the SCN is encoded in a complex firing pattern that had previously been overlooked, the researchers concluded. Forger and U-M graduate student Casey Diekman, along with Dr. Mino Belle and Hugh Piggins of the University of Manchester in England, report their findings in the Oct. 9 edition of Science.

To test predictions made by Forger and Diekman's mathematical model, the British scientists collected data on firing patterns from more than 400 mouse SCN cells. The U-M scientists then plugged the experimental results into their model and found that "the experimental data were almost exactly what the model had predicted," Forger said.

Though the experiments were done with mice, Forger said it's likely that the same mechanism is at work in humans, since timekeeping systems are similar in all mammals.

The SCN contains both clock cells (which express a gene call per1) and non-clock cells. For years, circadian-biology researchers have been recording electrical signals from a mix of both types of cells. That led to a misleading picture of the clock's inner workings.

But Forger's British colleagues were able to separate clock cells from non-clock cells by zeroing in on the ones that expressed the per1 gene. Then they recorded electrical signals produced exclusively by those clock cells. The pattern that emerged bolstered the audacious new theory.

"This is a perfect example of how a mathematical model can make predictions that are completely at odds with the prevailing views yet, upon further experimentation, turn out to be dead-on," Forger said.

The researchers found that during the day, SCN cells expressing per1 sustain an electrically excited state but do not fire. They fire for a brief period around dusk, then remain quiet throughout the night before releasing another burst of activity around dawn. This firing pattern is the signal, or code, the brain sends to the rest of the body so it can keep time.

"The old theory was that the cells in the SCN which contain the clock are firing fast during the day but slow at night. But now we've shown that the cells that actually contain the clock mechanism are silent during the day, when everybody thought they were firing fast," Diekman said.

Piggins said the findings "force us to completely reassess what we thought we knew about electrical activity in the brain's circadian clock." In addition, the results demonstrate the importance of interdisciplinary collaborative research, he said.

"This work also raises important questions about whether the brain acts in an analog or a digital way," Belle said.

Learning To Talk Changes How Speech Is Heard: 'Sound Of Learning' Unlocked By Linking Sensory And Motor Systems

Learning to talk also changes the way speech sounds are heard, according to a new study published in Proceedings of the National Academy of Sciences by scientists at Haskins Laboratories, a Yale-affiliated research laboratory. The findings could have a major impact on improving speech disorders.

"We've found that learning is a two-way street; motor function affects sensory processing and vice-versa," said David J. Ostry, a senior scientist at Haskins Laboratories and professor of psychology at McGill University. "Our results suggest that learning to talk makes it easier to understand the speech of others."

As a child learns to talk, or an adult learns a new language, Ostry explained, a growing mastery of oral fluency is matched by an increase in the ability to distinguish different speech sounds. While these abilities may develop in isolation, it is possible that learning to talk also changes the way we hear speech sounds.

Ostry and co-author Sazzad M. Nasir tested the notion that speech motor learning alters auditory perceptual processing by evaluating how speakers hear speech sounds following motor learning. They simulated speech learning by using a robotic device, which introduced a subtle change in the movement path of the jaw during speech.

To assess speech perception, the participants listened to words one at a time that were taken from a computer-produced continuum between the words "had" and "head." In the speech learning phase of the study, the robot caused the jaw to move in a slightly unusual fashion. The learning is measured by assessing the extent to which participants correct for the unusual movement.

"Its like being handed a two-pound weight for the first time and being asked to make a movement, it's uncomfortable at first, but after a while, the movement becomes natural," said Ostry. "In growing children, the nervous system has to adjust to moving vocal tract structures that are changing in size and weight in order to produce the same words. Participants in our study are learning to return the movement to normal in spite of these changes. Eventually our work could have an impact on deviations to speech caused by disorders such as stroke and Parkinson's disease."

"Our study showed that speech motor learning altered the perception of these speech sounds. After motor learning, the participants heard the words differently than those in the control group," said Ostry. "One of the striking findings is that the more motor learning we observed, the more their speech perceptual function changed."

Ostry said that future research will focus on the notion that sensory remediation may be a way to jumpstart the motor system.

The team previously found that the movement of facial muscles around the mouth plays an important role not only in the way the sounds of speech are made, but also in the way they are heard.

Haskins Laboratories was founded in 1935 by the late Dr. Caryl P. Haskins. This independent research institute has been in New Haven, Connecticut since 1970 when it formalized affiliations with Yale University and the University of Connecticut. The Laboratories' primary research focus is on the science of the spoken and written word.

Short Heels Make Elite Sprinters Super Speedy: Longer Toes, Unique Ankle Structure Aid Sprinters

Longer toes and a unique ankle structure provide sprinters with the burst of acceleration that separates them from other runners, according to biomechanists.

"At the start of a sprint the only way a runner can speed up is through the reaction force that results from the action of leg muscles pushing on the ground," said Stephen Piazza, associate professor of kinesiology, Penn State. "Long toes provide sprinters the advantage of maintaining maximum contact with the ground just a little bit longer than other runners."

Piazza and his colleague Sabrina S. M. Lee, former Penn State graduate student now a post-doctoral fellow at Simon Fraser University, Vancouver, Canada, studied the muscle architecture of the foot and ankle to look at the differences between sprinters and non-sprinters.

They matched 12 collegiate sprinters with 12 non-athletes of the same height. They measured the distance between the heel and the end of the toes and used ultrasound imaging to measure the sliding of the Achilles tendon during ankle motion, from which the leverage of the tendon can be calculated.

"What we found was that the lever arms (distance between the tendon and center of rotation of the ankle) were significantly shorter -- about 25 percent shorter -- in sprinters," said Piazza, whose findings appeared recently in the Journal of Experimental Biology. "This difference might be explained by a tradeoff between leverage and muscle force-generating capacity."

Because the lever arms are shorter, the muscles shorten less for the same joint rotation. If muscles shorten less, they shorten more slowly, which helps them to produce greater force that more than compensates for the reduced leverage.

While there is little published work on foot shapes and sprinting, previous work on animals suggests that ostriches, greyhounds and cheetahs have feet built for sprinting.

To understand the kind of human foot that would produce a similar sprinting advantage, the researchers developed a simple computer model that could analyze the physiological data they had collected earlier.

"We wanted to see how much acceleration we could get out of the model when we changed the tendon lever arm and the length of the toes," said Piazza. "What we found is that when the Achilles tendon lever arm is the shortest and the toes are longest, we get the greatest acceleration."

Piazza cites other recent research suggesting that shorter toes in modern humans could be an evolutionary adaptation for efficient distance running.

"Maybe our ancestors with longer toes were better sprinters. Or maybe longer toes were selected for at a time when navigating in trees was more important and our toes became shorter as endurance running became more important for our survival," he added.

The Penn State researcher cautions that while the study could be a piece of the puzzle in determining who could potentially be a good sprinter, other physiological components such as body type, cardiovascular physiology and muscle fiber types should also be taken into account.

It is also unclear whether sprinting ability is congenital or whether training can influence the shape of bones in the foot.

"It is not too far-fetched to think that training can help accentuate the shape of the bone," said Piazza. "But if sprinters' skeletal characteristics were shown to be immutable, it would support the coaches' adage that sprinters are born and not made."

The National Science Foundation funded this work.

NASA's Fermi Telescope Detects Gamma Rays From 'Star Factories' In Other Galaxies

Nearby galaxies undergoing a furious pace of star formation also emit lots of gamma rays, say astronomers using NASA's Fermi Gamma-ray Space Telescope. Two so-called "starburst" galaxies, plus a satellite of our own Milky Way galaxy, represent a new category of gamma-ray-emitting objects detected both by Fermi and ground-based observatories.

"Starburst galaxies have not been accessible in gamma rays before," said Fermi team member Seth Digel, a physicist at SLAC National Accelerator Laboratory in Menlo Park, Calif. "Most of the galaxies Fermi sees are exotic and distant blazars, which produce jets powered by matter falling into enormous black holes. But these new galaxies are much closer to us and much more like our own."

Gamma rays are the most energetic form of light. Fermi has detected more than a thousand point sources and hundreds of gamma-ray bursts, but the satellite also detects a broad glow that roughly follows the plane of our galaxy. This diffuse gamma-ray emission results when fast-moving particles called cosmic rays strike galactic gas or even starlight.

Cosmic rays are hyperfast electrons, positrons, and atomic nuclei moving at nearly the speed of light. But, although Earth is constantly bombarded by these particles, their origin remains a mystery nearly a century after their discovery. Astronomers suspect that the rapidly expanding shells of exploded stars somehow accelerate cosmic ray particles to their fantastic energy.

"For the first time, we're seeing diffuse emission from star-forming regions in galaxies other than our own," noted Jürgen Knödlseder, a Fermi collaborator at the Center for the Study of Space Radiation in Toulouse, France. He spoke to reporters today at the 2009 Fermi Symposium, a Washington gathering of hundreds of astrophysicists involved in the Fermi mission and related studies. The meeting continues through Nov. 5.

Knödlseder revealed an image captured by Fermi's Large Area Telescope (LAT) of a star-forming region known as 30 Doradus within the Large Magellanic Cloud (LMC). Located 170,000 light-years away in the southern constellation Dorado, the LMC is the largest of several small satellite galaxies that orbit our own.

More stars form in the 30 Doradus "star factory" than in any similar location in the Milky Way. "The region is an intense source of gamma rays, and the diffuse emission we see with Fermi follows the glowing gas we see in visible light," Knödlseder explained.

The region lights up in gamma rays for the same reason the Milky Way does -- because cosmic rays strike gas clouds and starlight. But Fermi shows that the LMC's brightest diffuse emission remains close to 30 Doradus and doesn't extend across the galaxy. This implies that the stellar factory itself is the source of the cosmic rays producing the glow.

"Star-forming regions produce lots of massive, short-lived stars, which explode when they die," Digel said. "The connection makes sense."

"The tangled magnetic fields near 30 Doradus probably confine the cosmic rays to their acceleration sites," Knödlseder said.

Fermi's LAT sees diffuse emission from the starburst galaxies M82 and NGC 253, both of which were also seen this year by ground-based observatories sensitive to gamma rays hundreds of times more energetic than the LAT can detect. They do this by imaging faint flashes in the upper atmosphere caused by the absorption of gamma rays carrying trillions of times the energy of visible light.

"The core of M82 forms stars at a rate ten times greater than the entire Milky Way galaxy," said Niklas Karlsson, a postdoctoral fellow at Adler Planetarium in Chicago. He is also a member of the science team for VERITAS, an array of gamma-ray telescopes in Arizona that detected M82, which lies 12 million light-years away in the constellation Ursa Major.

"These very-high-energy gamma rays probe physical processes in other galaxies that will help us understand how and where cosmic rays become accelerated," Karlsson explained.

"Our sensitivity to gamma-rays -- both in space and on the ground -- has increased enormously thanks to Fermi and observatories like VERITAS," Digel said. "This is opening up the detailed study of high-energy processes in galaxies very close to home." NASA's Fermi Gamma Ray Space Telescope is an astrophysics and particle physics partnership, developed in collaboration with the U.S. Department of Energy, along with important contributions from academic institutions and partners in France, Germany, Italy, Japan, Sweden and the United States.

Newly Discovered Ankylosaur Dinosaur Is 'Biological Version Of An Army Tank'

A husband and wife team of American paleontologists has discovered a new species of dinosaur that lived 112 million years ago during the early Cretaceous of central Montana.

The new dinosaur, a species of ankylosaur, is documented in the October issue of the Canadian Journal of Earth Sciences. Ankylosaurs are the biological version of an army tank. They are protected by a plate-like armour with two sets of sharp spikes on each side of the head, and a skull so thick that even 'raptors' such as Deinonychus could leave barely more than a scratch.

Bill and Kris Parsons, Research associates of the Buffalo Museum of Science, found much of the skull of the newly described Tatankacephalus cooneyorum resting on the surface of a hillside in 1997. Because the skull was 90% complete, it was possible to justify this fossil as a new species.

"This is the first member of Ankylosauridae to be found within the Early Cretaceous Cloverly Geologic Formation," said Bill Parsons, who characterized the fossil as a transitional evolutionary form between the earlier Jurassic ankylosaurs and the better known Late Cretaceous ankylosaurs.

The skull is heavily protected by two sets of lateral horns, two thick domes at the back, and smaller thickenings around the nasal region. "Heavy ornamentation and horn-like plates would have covered most of the dorsal surface of this dinosaur" said Bill Parsons.

"For years, Bill and Kris have been collecting fossils from a critical time in Earth's history, and their hard work has paid off," said Lawrence Witmer, professor of paleontology at Ohio University who was not involved with this study. "This is a really important find and gives us a clearer view of the evolution of armored dinosaurs. But this is just the first; I'm sure, of what will be a series of important discoveries from this team."

Parsons also illustrated the dermal armour of this new species based on the theory by Museum of the Rockies paleontologist John R. Horner that there was an outer keratinous sheathing on it as found in modern turtle shells and bird beaks. In his new reconstruction, Parsons suggests that Tatankacephalus exhibited complex and colorful patterns rather than the dull appearance suggested in earlier ankylosaur portraits. "According to Horner's theory, many other dinosaurs also had this kind of sheathing and also may have been diversely colored," said Parsons.

As to its name, the broad, short horns on the back of its skull resemble the horns found on a modern buffalo skull and Tatankacephalus loosely translates as 'Buffalo head.' Parsons also noted, "of course any further allusions to the city of Buffalo are completely intentional as well."

New Analyses Of Dinosaur Growth May Wipe Out One-third Of Species

Paleontologists from the University of California, Berkeley, and the Museum of the Rockies have wiped out two species of dome-headed dinosaur, one of them named three years ago -- with great fanfare -- after Hogwarts, the school attended by Harry Potter.

Their demise comes after a three-horned dinosaur, Torosaurus, was assigned to the dustbin of history last month at the Society of Vertebrate Paleontology meeting in the United Kingdom, the loss in recent years of quite a few duck-billed hadrosaurs and the probable disappearance of Nanotyrannus, a supposedly miniature Tyrannosaurus rex.

These dinosaurs were not separate species, as some paleontologists claim, but different growth stages of previously named dinosaurs, according to a new study. The confusion is traced to their bizarre head ornaments, ranging from shields and domes to horns and spikes, which changed dramatically with age and sexual maturity, making the heads of youngsters look very different from those of adults.

"Juveniles and adults of these dinosaurs look very, very different from adults, and literally may resemble a different species," said dinosaur expert Mark B. Goodwin, assistant director of UC Berkeley's Museum of Paleontology. "But some scientists are confusing morphological differences at different growth stages with characteristics that are taxonomically important. The result is an inflated number of dinosaurs in the late Cretaceous."

Goodwin and John "Jack" Horner of the Museum of the Rockies at Montana State University in Bozeman, are the authors of a new paper analyzing North American dome-headed dinosaurs that appeared this week in the public access online journal PLoS One.

Unlike the original dinosaur die-off at the end of the Cretaceous period 65 million years ago, this loss of species is the result of a sustained effort by paleontologists to collect a full range of dinosaur fossils -- not just the big ones. Their work has provided dinosaur specimens of various ages, allowing computed tomography (CT) scans and tissue study of the growth stages of dinosaurs.

In fact, Horner suggests that one-third of all named dinosaur species may never have existed, but are merely different stages in the growth of other known dinosaurs.

"What we are seeing in the Hell Creek Formation in Montana suggests that we may be overextended by a third," Horner said, a "wild guess" that may hold true for the various horned dinosaurs recently discovered in Asia from the Cretaceous. "A lot of the dinosaurs that have been named recently fall into that category."

The new paper, published online Oct. 27, contains a thorough analysis of three of the four named dome-headed dinosaurs from North America, including Pachycephalosaurus wyomingensis, the first "thick-headed" dinosaur discovered. After that dinosaur's description in 1943, many speculated that male pachycephalosaurs used their bowling ball-like domes to head-butt one another like big-horn sheep, though Goodwin and Horner disproved that notion in 2004 after a thorough study of the tissue structure of the dome.

Many paleontologists now realize that the elaborate head ornaments of dinosaurs, from the huge bony shield and three horns of Triceratops to the coxcomb-like head gear of some hadrosaurs, were not for combat, but served the same purpose as feathers in birds: to distinguish between species and indicate sexual maturity.

"Dinosaurs, like birds and many mammals, retain neoteny, that is, they retain their juvenile characteristics for a long period of growth," Horner said, "which is a strong indicator that they were very social animals, grouping in flocks or herds with long periods of parental care."

These head ornaments, which probably had horny coverings of keratin that may have been brightly-colored as they are in many birds, started growing when these dinosaurs reached about half their adult size, and were remodeled as these dinosaurs matured, continuing to change shape even into adulthood and old age, according to the researchers.

In the new paper, Horner and Goodwin compared the bone structures of Pachycephalosaurus with that of a domeheaded dinosaur, Stygimoloch spinifer, discovered in Montana by UC Berkeley paleontologists in 1973, and a dragon-like skull discovered in South Dakota and named in 2006 as a new species, Dracorex hogwartsia.

With the help of CT scans and microscopic analysis of slices through the bones of Pachycephalosaurus and Stygimoloch, the team concluded that Stygimoloch, with its high, narrow dome, growing tissue and unfused skull bones, was probably a pachycephalosaur subadult, in a stage just before sexual maturity.

Dracorex is one of a kind, and thus unavailable for dissection, but morphological analysis indicates it is a juvenile that hasn't yet formed a dome, although the top of its skull shows thickening suggestive of an emerging dome.

"Dracorex's flat skull, nodules on the front end and small spikes on back, and thickened but undomed frontoparietal bone all confirm that, ontogenetically, it is a juvenile Pachycephalosaurus," Goodwin said.

Comparison of these skulls to other fossils in the hands of private collectors confirm the conclusions, they said. In all, they looked at 21 dome-headed dinosaur skulls and cranial elements from North America.

The key to this analysis, Horner said, was years of field work in Montana by his team and Goodwin's in search of fossils of all sizes.

"We have gone out in the Hell Creek Formation for 11 years doing nothing but collecting absolutely everything we could find, which is the kind of collecting that is required," he said. "If you think about Triceratops, people had collected for 100 years and still hadn't found any juveniles. And we went out and spent 11 years collecting everything, and we found all kinds of them."

"Early paleontologists recognized the distinction between adults and juveniles, but people have lost track of looking at ontogeny -- how the individual develops -- when they discover a new fossil," Goodwin said. "Dinosaurs are not mammals, and they don't grow like mammals."

In fact, the so-called metaplastic bone on the heads of horned dinosaurs grows and dissolves, or resorbs, throughout life like no other bone, Horner said, and is reminiscent of the growth and loss of horns today in elk and deer. In earlier studies, Horner and Goodwin found dramatic remodeling of metaplastic bone in Triceratops, which led to their subsequent focus on dome-headed dinosaurs.

"Metaplastic bones get long and shorten, as in Triceratops, where the horn orientation is backwards in juveniles and forward in adults," Horner said. Even in older specimens, such as the fossil previously named Torosaurus, bone in the face shield resorbs to create holes along the margin. John Scannella, Horner's student at Montana State, presented a paper reclassifying Torosaurus as an old Triceratops at the Society for Vertebrate Paleontology meeting in Bristol, U.K., on Sept. 25.

"In order for that huge amount of bone to move, there has to be a lot of deposition and resorption," Horner said.

Horner and Goodwin continue to search for dinosaur fossils in the Hell Creek Formation, which is rich in Triceratops, dome-headed dinosaurs, hadrosaurs and tyrannosaurs. Analysis of growth stages in these taxa will have implications for other horned dinosaurs that are being uncovered in Asia and elsewhere.

"There are other horned dinosaurs I think may be over split," that is, split into too many new species rather than being lumped together as one species, Goodwin said.

The work was supported by grants from the UC Museum of Paleontology and the Museum of the Rockies.

Opening Up A Colorful Cosmic Jewel Box

Star clusters are among the most visually alluring and astrophysically fascinating objects in the sky. One of the most spectacular nestles deep in the southern skies near the Southern Cross in the constellation of Crux.

The Kappa Crucis Cluster, also known as NGC 4755 or simply the "Jewel Box" is just bright enough to be seen with the unaided eye. It was given its nickname by the English astronomer John Herschel in the 1830s because the striking colour contrasts of its pale blue and orange stars seen through a telescope reminded Herschel of a piece of exotic jewellery.

Open clusters [1] such as NGC 4755 typically contain anything from a few to thousands of stars that are loosely bound together by gravity. Because the stars all formed together from the same cloud of gas and dust their ages and chemical makeup are similar, which makes them ideal laboratories for studying how stars evolve.

The position of the cluster amongst the rich star fields and dust clouds of the southern Milky Way is shown in the very wide field view generated from the Digitized Sky Survey 2 data. This image also includes one of the stars of the Southern Cross as well as part of the huge dark cloud of the Coal Sack [2].

A new image taken with the Wide Field Imager (WFI) on the MPG/ESO 2.2-metre telescope at ESO's La Silla Observatory in Chile shows the cluster and its rich surroundings in all their multicoloured glory. The large field of view of the WFI shows a vast number of stars. Many are located behind the dusty clouds of the Milky Way and therefore appear red [3].

The FORS1 instrument on the ESO Very Large Telescope (VLT) allows a much closer look at the cluster itself. The telescope's huge mirror and exquisite image quality have resulted in a brand-new, very sharp view despite a total exposure time of just 5 seconds. This new image is one of the best ever taken of this cluster from the ground.

The Jewel Box may be visually colourful in images taken on Earth, but observing from space allows the NASA/ESA Hubble Space Telescope to capture light of shorter wavelengths than can not be seen by telescopes on the ground. This new Hubble image of the core of the cluster represents the first comprehensive far ultraviolet to near-infrared image of an open galactic cluster. It was created from images taken through seven filters, allowing viewers to see details never seen before. It was taken near the end of the long life of the Wide Field Planetary Camera 2 ― Hubble's workhorse camera up until the recent Servicing Mission, when it was removed and brought back to Earth. Several very bright, pale blue supergiant stars, a solitary ruby-red supergiant and a variety of other brilliantly coloured stars are visible in the Hubble image, as well as many much fainter ones. The intriguing colours of many of the stars result from their differing intensities at different ultraviolet wavelengths.

The huge variety in brightness of the stars in the cluster exists because the brighter stars are 15 to 20 times the mass of the Sun, while the dimmest stars in the Hubble image are less than half the mass of the Sun. More massive stars shine much more brilliantly. They also age faster and make the transition to giant stars much more quickly than their faint, less-massive siblings.

The Jewel Box cluster is about 6400 light-years away and is approximately 16 million years old.


Notes

[1] Open, or galactic, star clusters are not to be confused with globular clusters ― huge balls of tens of thousands of ancient stars in orbit around our galaxy and others. It seems that most stars, including our Sun, formed in open clusters.

[2] The Coal Sack is a dark nebula in the Southern Hemisphere, near the Southern Cross, that can be seen with the unaided eye. A dark nebula is not the complete absence of light, but an interstellar cloud of thick dust that obscures most background light in the visible.

[3] If the light from a distant star passes through dust clouds in space the blue light is scattered and absorbed more than the red. As a result the starlight looks redder when it arrives on Earth. The same effect creates the glorious red colours of terrestrial sunsets.

HIV Tamed By Designer 'Leash'

Researchers have shown how an antiviral protein produced by the immune system, dubbed tetherin, tames HIV and other viruses by literally putting them on a leash, to prevent their escape from infected cells. The insights, reported in the October 30th issue of the journal Cell, a Cell Press publication, allowed the research team to design a completely artificial protein -- one that did not resemble native tetherin in its sequence at all -- that could nonetheless put a similar stop to the virus.

 

"Tetherin is essentially a rod with anchors at either end that are critical for its function," says Paul Bieniasz of Howard Hughes Medical Institute and the Aaron Diamond AIDS Research Center at The Rockefeller University. Either one of those anchors gets incorporated into the envelope surrounding HIV or other viruses as they bud through the plasma membrane of an infected cell. "One anchor gets into the virus and the other in the cell membrane to inevitably form a tether.
"We showed we could design a completely different protein with the same configuration -- a rod with lipid anchors at either end -- and it worked very well," he continued. The finding helped to confirm that tetherin is capable of acting all on its own, he added.
They also explain tetherin's broad specificity to protect against many viruses. "It is just targeting lipids," Bieniasz said. "It's not about viral proteins." That's conceptually important, he continued, because there is no specific interaction between tetherin and any viral protein, which makes it a more difficult problem for viruses to evolve resistance. Rather than tweaking an existing protein-coding gene, "the virus has to make the more difficult adjustment of acquiring a new gene antagonist [of tetherin]."
Unfortunately, many viruses have managed to do just that. In the case of HIV, a protein called Vpu counteracts tetherin. They now show it does so by sequestering the host protein, which prevents its incorporation into the virus. The new insight into tetherin's and Vpu's modes of action, however, may lead to the development of Vpu blockers that could free up the innate host defense and inhibit HIV's spread, Bieniasz suggests.
Bieniasz said there is some possibility that tetherin exists in different forms that might explain differences among people in the progression of HIV or other viral infections. However, the only common variation they've seen in the tetherin gene so far does not appear to affect its function. The tetherin sequence does vary quite a lot from one species to the next, he added, as is often the case due to strong selection when host defense genes meet viral inhibitors.
To place the findings in context, Bieniasz says it is worth noting that tetherin is encoded by just one of more than 900 genes that get switched "on" in response to interferon, a cell signaling protein of the immune system.
"There are hundreds of interferon-induced genes," he said. "The functions are known for only a very small number -- less than a dozen. There are potentially a large number of antiviral mechanisms we still know nothing about."
Going forward, his team intends to look more closely at many of those others, and Bieniasz suspects more surprising mechanisms will be in store.
The researchers include David Perez-Caballero, The Rockefeller University, New York, NY; Trinity Zang, The Rockefeller University, New York, NY, Howard Hughes Medical Institute, Aaron Diamond AIDS Research Center, New York, NY; Alaleh Ebrahimi, The Rockefeller University, New York, NY; Matthew W. McNatt, The Rockefeller University, New York, NY; Devon A. Gregory, University of Missouri School of Medicine, Columbia, MO; Marc C. Johnson, University of Missouri School of Medicine, Columbia, MO; and Paul D. Bieniasz, The Rockefeller University, New York, NY, Howard Hughes Medical Institute, Aaron Diamond AIDS Research Center, New York, NY.
 

Polarized Peepers: Crustacean's Eyes Surpass Man-Made Optical Devices in Manipulating Light

A fierce crustacean known as the peacock mantis shrimp has eyes so refined they can perceive polarized light, including information that is invisible to nearly every other member of the animal kingdom. Not only can the ocean dweller extract polarization information from light, it can do so when the light is circularly polarized—an ability unknown outside a few species of the order of stomatopods to which the peacock mantis belongs.

Unlike linearly polarized light, in which the electric field oscillates along a plane, circularly polarized light's field twists like a spiral spring as the ray propagates. Such light is not commonly reflected from animal bodies and so was long dismissed as a virtual nonfactor in physiology, but research last year showed that some stomatopods have the ability to discriminate circular polarization. A paper published online October 25 in Nature Photonics unpacks the mechanism behind the mantis shrimp's ability and concludes that its eyes handle circularly polarized light more effectively than man-made optical devices do. (Scientific American is part of the Nature Publishing Group.)

The peacock mantis, or Odontodactylus scyllarus, packs a surprisingly powerful punch for its size. The crustacean, which ranges from three to 18 centimeters in length, is capable of shattering the glass of an aquarium with a blow from its forelimb, says Roy Caldwell, a University of California, Berkeley, biologist who did not participate in the new research. "We have had a couple cases where animals have hit a pane of glass dead in the center and there was a massive explosion," Caldwell says.

But the creature is physiologically remarkable in at least one other way: The compound eye of the peacock mantis, the new study's authors found, harbors a natural quarter-wave retarder, a sort of filter that converts circularly polarized light to linearly polarized light, which then activates receptors below. "Biologically, this is unique," says study co-author Thomas Cronin, a professor of biological sciences at the University of Maryland, Baltimore County. "There is nothing else known anywhere in biology" that enables detection of circularly polarized light, he adds.

The stomatopods reflect circularly polarized light from their bodies, so their ability to detect such light—and to parse clockwise from counterclockwise polarization—likely plays a role in signaling or identification. In some stomatopod species, reflection of circularly polarized light is sex-specific, which could play a role in sexual signaling or mate selection.

Wave retarders work by refracting light differently depending on the angle of its polarization, delaying one wave component of a light wave relative to the other. "If it's just the right degree of delay, which is one-quarter wave or 90 degrees phase, that converts circularly polarized light to linearly polarized," Cronin explains. But unlike wave retarders available commercially, which are tuned for specific wavelengths (and hence colors) of visible light, the wave plate in the O. scyllarus eye performs almost identically across the visible spectrum.

The mantis shrimp's eye, Cronin explains, "works on a principle that is not used currently but could be used in manufacturing systems"—balancing the optical properties of the eye structure with those of the lipid molecules that fill the structure. "The two have different wavelength functions—they have different curves of changing retardance with wavelength—and so the animal trades them off," Cronin says. "It trades off structure against material to cancel out the two variations."

Sonja Kleinlogel, a biologist at the Max Planck Institute of Biophysics in Frankfurt am Main, Germany, points out that she and a colleague published a similar analysis last year in the journal PLoS ONE—an article that she was surprised to see omitted from the references section of the new paper. Nevertheless, she is pleased to see the subject advanced, noting that the research "is the first to look at the detailed structure" of the cells that act as quarter-wave retarders and to compare their efficacy with man-made analogues. U.C. Berkeley's Caldwell concurs, noting that the unique capability of the stomatopod eye had been described but "how that actually was done was pretty much a mystery."

"We didn't know anything about the operating principle of the retarder," Cronin says. "It wasn't like anything we had seen in the lab."

Link Between Alcohol And Cancer Explained: Alcohol Activates Cellular Changes That Make Tumor Cells Spread

Alcohol consumption has long been linked to cancer and its spread, but the underlying mechanism has never been clear. Now, researchers at Rush University Medical Center have identified a cellular pathway that may explain the link.

In a study published in a recent issue of Alcoholism: Clinical and Experimental Research, the researchers found that alcohol stimulates what is called the epithelial-to-mesenchymal transition, in which run-of-the-mill cancer cells morph into a more aggressive form and begin to spread throughout the body.

"Our data are the first to show that alcohol turns on certain signals inside a cell that are involved in this critical transition," said Christopher Forsyth, PhD, assistant professor of medicine and biochemistry at Rush University Medical Center and lead author of the study.

The epithelial-to-mesenchymal transition is a hot area of research right now, implicated in the process whereby cancer cells become metastatic. A large body of laboratory and clinical research suggests that it plays a key role in making cancer cells aggressive.

"Cancer cells become dangerous when they metastasize," Forsyth said. "Surgery can remove a tumor, but aggressive tumor cells invade tissues throughout the body and take over. If we can thwart this transition, we can limit cancer's toll."

The researchers treated colon and breast cancer cell lines with alcohol and then looked for the biochemical hallmarks of the epithelial-to-mesenchymal transition, including evidence of a transcription factor called Snail and of the receptor for epidermal growth factor. Snail controls the epithelial-to-mesenchymal transition; when overexpressed in mice, it induces the formation of multiple tumors. Epidermal growth factor is required by many cancer cells. "They need lots of it," Forsyth said. "They are addicted to it."

Laboratory tests showed that alcohol activated both these and other biochemicals characteristic of the epithelial-to-mesenchymal transition. Tests also demonstrated that the alcohol-treated cells had lost their tight junctions with adjacent cells, a preparation for migrating, as metastatic cells do.

In addition, Forsyth and his colleagues found that the same roster of biomarkers was activated in normal intestinal cells treated with alcohol, suggesting that alcohol not only worsens the profile of existing cancer cells but also may initiate cancer by stimulating the epithelial-to-mesenchymal transition.

Genome Of Microbe Silently Shaping Ecology Of Ocean Dead Zones Described

Among the many changes in the ocean is the expansion of oxygen-deficient or oxygen minimum zones (OMZs), also known as dead zones, which affect the processes by which carbon is captured and sequestered on the seafloor and alter the microbial activities that impact the rate and magnitude of ocean carbon sequestration. Despite the importance of these effects, very little is known about the metabolism of OMZ microbes and how they respond to environmental changes.

In the Oct. 23 issue of the journal Science, researchers from the University of British Columbia and the U.S. Department of Energy Joint Genome Institute (DOE JGI) describe the metagenome of an abundant but uncultivated microbe, known as SUP05, that is silently helping to shape the ecology of OMZs worldwide. Researchers studied the microbe in Saanich Inlet, a fjord on the coast of Vancouver Island, British Columbia, Canada. The fjord undergoes a seasonal cycle of stratification and deep water renewal, creating strong water column gradients that make it an ideal "living lab" to study microbial communities adapted and specialized to thrive under low oxygen conditions such as those found in OMZs. To chart the SUP05 metagenome, genetic material was recovered directly from environmental samples encompassing the entire microbial community of Saanich Inlet during different stages of water column stratification and deep-water renewal.

"To our surprise the most abundant organism in the oxygen-depleted waters was this SUP05 bug," said the paper's senior author Steven Hallam, Assistant Professor at the University of British Columbia. "We obtained enough DNA sequence coverage from the community of microbes to actually assemble a continuous stretch representing what we are calling the SUP05 metagenome -- it's a composite of the entire SUP05 population spanning the various environmental samples that we sequenced."

Susannah Tringe, a metagenomics scientist at the DOE JGI, said that the OMZs are sinks for an essential nutrient that marine organisms need to survive--nitrogen--as well as sources for the greenhouse gases methane and nitrous oxide. "By studying the genomes of the uncultivated microbes found in OMZs, we can better understand how they participate in global geochemical cycles such as the carbon and nitrogen cycles," she said.

Hallam described SUP05 as a paradoxical organism. "Based on genomic analysis and field observations, it provides important ecosystem services but it also produces byproducts that may have negative consequences with respect to climate change," he said. "Specifically, SUP05 removes toxic sulfides from the water, and fixes carbon dioxide but we also think it's producing nitrous oxide, which is a more potent greenhouse gas than either carbon dioxide or methane."

The researchers found that SUP05 is closely related to sulfur-eating gill symbionts of deep sea clams and mussels although unlike them, it utilizes nitrate rather than oxygen in its energy metabolism. A comparative analysis of SUP05 and symbiont genomes also revealed that 35 percent of the SUP05 genome is unique, involved in helping the bacteria adapt to changing environmental conditions such as the seasonal increase and decrease of oxygen levels in Saanich Inlet, and the shifting balance of the nitrate and sulfide levels that are its key energy resources.

"As habitat range expands due to global warming, blooming SUP05 populations have the potential to help offset rising carbon dioxide levels that ultimately lead to ocean acidification," Hallam said. He added that SUP05 and its relatives will become increasingly important agents as OMZ expansion and intensification continues to unfold, providing researchers with a biological indicator useful in monitoring the changing state of the global ocean.

The researchers plan to do further time course and metabolic monitoring studies in Saanich Inlet in conjunction with the Victoria Experimental Network Under the Sea (VENUS) cabled observatory program to better constrain the ecological roles of SUP05 and other, less abundant community members. Additionally, they hope to use the time-resolved studies in Saanich Inlet as a basis for comparison in the context of another CSP project of Hallam's that was approved earlier this year and focuses on an extensive OMZ in the eastern North Pacific Ocean.

"Just as cyanobacteria play an essential role in producing atmospheric oxygen; in future oceans this could be one of those organisms that play similarly integral roles, albeit with different ecological outcomes," Hallam said. An overview of the project can be viewed at: http://www.cmde.science.ubc.ca/hallam/index.php.

The project is part of the DOE JGI's Community Sequencing Program established in 2004 to take on large-scale genomics efforts in support of DOE mission areas, including characterizing the biological and environmental processes involved in carbon cycling.

Biofuel Displacing Food Crops May Have Bigger Carbon Impact Than Thought

A report examining the impact of a global biofuels program on greenhouse gas emissions during the 21st century has found that carbon loss stemming from the displacement of food crops and pastures for biofuels crops may be twice as much as the CO2 emissions from land dedicated to biofuels production. The study, led by Marine Biological Laboratory (MBL) senior scientist Jerry Melillo, also predicts that increased fertilizer use for biofuels production will cause nitrous oxide emissions (N2O) to become more important than carbon losses, in terms of warming potential, by the end of the century.

Using a global modeling system that links economic and biogeochemistry data, Melillo, MBL research associate David Kicklighter, and their colleagues examined the effects of direct and indirect land-use on greenhouse gas emissions as the production of biofuels increases over this century. They report their findings in the October 22 issue of Science Express.

Direct land-use emissions are generated from land committed solely to bioenergy production. Indirect land-use emissions occur when biofuels production on cropland or pasture displaces agricultural activity to another location, causing additional land-use changes and a net increase in carbon loss.

No major countries currently include carbon emissions from biofuel-related land-use changes in their carbon loss accounting and there is concern about the practicality of including such losses in a system designed to reduce fossil-fuel emissions. Moreover, methods to assess indirect land-use emissions are controversial. All quantitative analyses to date have either ignored indirect emissions altogether, considered those associated from crop displacement from a limited area, confused indirect emissions with direct or general land-use emissions, or developed estimates based on a static framework of today's economy.

Using a modeling system that integrates global land-use change driven by multiple demands for land and that includes dynamic greenhouse-gas accounting, Melillo and his colleagues factored in a full suite of variables, including the potential of net carbon uptake from enhanced land management, N2O emissions from the increased use of fertilizer, environmental effects on carbon storage, and the economics of land conversion.

"Our analysis, which we think is the most comprehensive to date, shows that direct and indirect land-use changes associated with an aggressive global biofuels program have the potential to release large quantities of greenhouse gases to the atmosphere," says Melillo.

Melillo and his colleagues simulated two global land-use scenarios in the study. In Case 1, natural areas are converted to meet increased demand for biofuels production land. In Case 2, there is less willingness to convert land and existing managed land is used more intensely. Both scenarios are linked to a global climate policy that would control greenhouse gas emissions from fossil fuel sources to stabilize CO2 concentrations at 550 parts per million, a target often talked about in climate policy discussions. Under such a climate policy, fossil fuel use would become more expensive and the introduction of biofuels would accelerate, ultimately increasing the size of the biofuels industry and causing additional effects on land use, land prices, and food and forestry production and prices.

The model predicts that, in both scenarios, land devoted to biofuels will become greater than the total area currently devoted to crops by the end of the 21st century. Case 1 will result in more carbon loss than Case 2, especially at mid-century. In addition, indirect land use will be responsible for substantially greater carbon losses (up to twice as much) than direct land use.

"Large greenhouse gas emissions from these indirect land-use changes are unintended consequences of a global biofuels program; consequences that add to the climate-change problem rather than helping to solve it," says Melillo "As our analysis shows, these unintended consequences are largest when the clearing of forests is involved."

In their model, Melillo and his colleagues also simulated N2O emissions from the additional fertilizer that will be required to grow biofuel crops in the future. They found that over the century, N2O emissions will surpass CO2 in terms of warming potential. By 2100, Melillo and his team estimate that in both study scenarios, biofuels production will account for more than half of the total N2O emissions from fertilizer. "Best practices for the use of nitrogen fertilizer, such as synchronizing fertilizer application with plant demand, can reduce N2O emissions associated with biofuels production," the scientists say.

Finding The ASX200 For Marine Ecosystems

Researchers are building the environmental equivalent of the ASX200 as a means of monitoring the health of Australian marine ecosystems.

The state of an ecosystem can be understood by measuring the right ecological characteristics, just as the ASX200 index shows the state of the Australian stock market by following 200 selected stocks.

CSIRO Wealth from Oceans Flagship scientist Dr Keith Hayes, one of the leaders of the project, says the difficult part is selecting which characteristics to track.

"Ecosystems respond to multiple pressures and threats in complex ways," Dr Hayes says.

"Identifying reliable and robust indicators of change from among the many species and characteristics of the system is a challenging task."

In a pilot project undertaken for the Department of Environment, Water, Heritage & the Arts (DEWHA) last year, CSIRO identified indicators for five 'key ecological features' named by the Commonwealth Government for the South-West Marine Region off Western Australia's coast.

Key ecological features chosen for this study included the Perth Canyon and large ocean eddies.

The indicators were different for each feature, but they included things like numbers of sea birds, area of coral compared to algae, and phytoplankton size.

Such indicators will help provide a firm scientific basis to feed into State of the Environment reporting at regional, national and international scales.

"Indicators are an important topic in environmental circles right now as they help us make better decisions about managing the environment," Commonwealth Environment Research Facilities (CERF) Marine Biodiversity Hub director Professor Nic Bax says.

CSIRO's approach uses qualitative modelling, supported by asset and threat mapping, to understand the drivers and pressures on ecological features, and to predict how these features will respond to changes over the next five to 15 years.

"Having selected a suite of indicators, the next challenge is to measure them and test how they are predicted to change when subject to processes such as climate change, industrial development and fishing pressures," Dr Hayes says.

"We were really pleased with the results of the pilot project and have now embarked on a two-year program with DEWHA to develop a list of ecological indicators for the rest of Australia's marine territory."

This new project is in addition to the current CERF program.

The work will be presented at the Nationally Relevant Environmental Monitoring workshop in Canberra October 20 and 21, which is hosted by CSIRO and the Marine Biodiversity Hub.

The Marine Biodiversity Hub is funded through the CERF program, an Australian Government initiative supporting research with a strong public good focus.

Sage-grouse Populations In US Intermountain West May Be Threatened By Energy Development, Study Predicts

A study released October 14th in the current issue of the peer-reviewed journal PLoS ONE sheds new light on oil and gas development potential in the Intermountain West. Maps accompanying the study show the impacts to greater sage-grouse populations in relation to potential energy development. If business as usual continues and more forward-thinking development strategies are not considered, sage-grouse populations will decline an additional 7 to 19 percent, the study's authors predict.

 

Prepared by scientists from The Nature Conservancy, the National Audubon Society, and the University of Montana, the study created a tool to understand the cumulative impacts of energy development on species in the West. Agencies can use the study's findings to determine how best to pursue energy independence while maintaining quality habitat that is critical to imperiled sage-grouse populations as well as a host of other species, including iconic big game of the West.

"This study illustrates how impacts to sensitive species, in this example sage-grouse, can be used to forecast biological trade-offs of newly proposed or ongoing development plans," said study co-author Dr. Kevin Doherty, Senior Ecologist at the National Audubon Society. "A 7-19% impact to sage-grouse in their eastern range from just one of the host of issues causing their declines, highlights the need for scientifically credible conservation planning tools to balance natural resource development with wildlife conservation."

Sage-grouse populations are considered indicators of ecosystem health and have been closely monitored by state game and fish agencies over the past decade. The greater sage-grouse is currently a candidate for Endangered Species listing -- a result that would have far reaching implications for a wide range of industries in the region.

"Sage-grouse are useful in prioritizing conservation because their abundance is indicative of large and intact shrub-dominated grasslands, the most endangered ecosystem in North America," said study co-author, Dr. David Naugle, Associate Professor, University of Montana. "Challenges with sage-grouse are a harsh reminder that the value of small-scale conservation actions may be negated if large-scale cumulative impacts are ignored."

The new study and its detailed maps of the Intermountain West indicates that future oil and gas drilling could impact up to 9.1 million acres of sagebrush shrub lands and 2.7 million acres of grasslands -- key sage-grouse habitat.

Global demand for energy has increased by more than 50 percent in the last half century, and a similar increase is projected between 2007 and 2030. Much of our domestic demand will be served by new exploration in the western US -- making the health of wildlife in the West, such as sage-grouse, an issue of national importance.

"The Conservancy and the Audubon Society have been jointly working with state and federal agencies to proactively identify areas of high biological value that may impact oil and gas operations," said study lead author Holly Copeland, spatial ecologist, with the Nature Conservancy in Wyoming. "Linking wildlife impacts with predictive oil and gas models will provide tools to decision makers charged with meeting the challenge of maintain healthy wildlife populations while responsibly developing domestic energy resources."

This study was supported with funding from the Liz Claiborne Art Ortenberg Foundation (http://www.lcaof.org), The Nature Conservancy, National Audubon Society, and the University of Montana.

Female Choice Benefits Mothers More Than Offspring

The great diversity of male sexual traits, ranging from peacock's elaborate train to formidable genitalia of male seed beetles, is the result of female choice. But why do females choose among males? In a new study published October 22 in Current Biology, researchers from Uppsala University found no support for the theory that the female choice is connected to "good genes".

The great diversity of male sexual traits, ranging from peacock's elaborate train to formidable genitalia of male seed beetles, is the result of female choice. But why do females choose among males? Remarkably, there is no consensus among biologists over the key question why females choose among males. At the heart of this debate lie two distinct possibilities -- that female choosiness is beneficial to the females themselves or that female choice traits are favoured because of 'good genes' that males contribute to female's offspring.

Across animal kingdom, females often resist male advances and only a small fraction of mating attempts result in copulations. Mating is costly, and one straightforward explanation for female resistance is that non-resistant females will suffer a reduction in their fitness. However, by resisting mating attempts, females are selecting for most 'persistent' males. Could it be that offspring of such 'persistent' males have higher fitness? If yes, female resistance can be viewed as a way of selecting for males that provide their offspring with 'good genes'.

We manipulated female choosiness by altering female ability to reject unwanted males in Adzuki beetle. Female beetles are constantly harassed by ardent males and thwart male mating attempts by vigorously kicking the unwanted suitors with their hind legs. We fitted females with prongs that reduced male ability to impose copulations. Alternatively, we reduced females' ability to resist copulations by shortening their hind legs. Females with increased ability to reject male mating attempts had much higher fitness than females whose resistance was reduced. What about the 'good genes'?

- We found no support for the idea that increased female resistance to mating results in sons that are more successful in competition with other males, or in more fertile daughters. Hence, female resistance is mostly beneficial to the female herself, while inadvertent selection for male 'persistence' plays a minor role, says Alexei Maklakov, who led the study.

Long Night Falls Over Saturn's Rings

As Saturn's rings orbit the planet, a section is typically in the planet's shadow, experiencing a brief night lasting from 6 to 14 hours. However, once approximately every 15 years, night falls over the entire visible ring system for about four days.

 

This happens during Saturn's equinox, when the sun is directly over Saturn's equator. At this time, the rings, which also orbit directly over the planet's equator, appear edge-on to the sun. During equinox, light from the sun hits the ring particles at very low angles, accenting their topography and giving us a three-dimensional view of the rings.

"The equinox is a very special geometry, where the sun is turned off as far as the rings themselves are concerned, and all energy comes from Saturn," said Dr. Michael Flasar of NASA's Goddard Space Flight Center in Greenbelt, Md.

During Saturn's latest equinox August 11, the rings reached a temperature of 382 degrees below zero Fahrenheit, the coldest yet observed, as seen by the Composite Infrared Spectrometer (CIRS) instrument on board the Cassini spacecraft in orbit around Saturn. CIRS was developed at NASA Goddard, and Flasar is the Principal Investigator for the instrument.

"The whole point of the CIRS observations of Saturn's rings, other than producing some cool pictures, is to learn something about the physical properties of the ring particles: their spin rates, how sluggish they are in storing and radiating heat (a diagnostic of size and composition), and their vertical distribution in the ring 'plane'," said Flasar.

Although the rings are thousands of miles wide, they are only about 30 feet thick. They are made of particles that are mostly water-ice. Scientists continue to debate the rings' origin and age. Some think they could be remnants of a shattered moon or captured comets, while others think they could have formed along with Saturn from the primordial disk of gas and dust that gave birth to our solar system.

"At first glance, Saturn's rings look broad and bland, but then we got close-up images from the Voyager flybys, and our reaction was: oh, my gosh, there's structure everywhere -- what's going on?" said Dr. Linda Spilker, of NASA's Jet Propulsion Laboratory (JPL), Pasadena, Calif.

Researchers have discovered that while most of the ring particles are as small as dust and pebbles, there are a few chunks as big as mountains, and even some small moons several miles across embedded in the rings. Instead of orderly orbiting around Saturn, the particles clump together and drift apart, and the rings ripple and warp under the gravitational influence of Saturn's swarm of more than 60 moons.

"The closer we look at the rings, the more complex they get," says Spilker, Deputy Project Scientist for the CASSINI mission and a Co-Investigator on CIRS. She is leading the instrument team's investigation of the rings.

"Because Saturn's rings are so extended, going out to more than twice Saturn's radius (from the cloud tops), the furthest rings get less heat from Saturn than the innermost rings, so the ring temperatures at equinox tend to fall off with distance from Saturn's center," said Flasar.

However, the CIRS team discovered that the A-ring -- the outermost of the wide, bright rings -- did not cool off as much as expected during the equinox. This might give clues about its structure and evolution. "One possibility is that the gravitational influence of moons outside the A-ring is stirring up waves in it," said Spilker. "These waves could be much higher than the typical thickness of the rings. Since the waves rise above the ring plane, material in the waves would still be exposed to sunlight during the equinox, which would warm up the A-ring more than expected."

"But we have to carefully test this idea with computer models to see if it produces the temperatures we observed with CIRS," adds Spilker. "That's the challenge with CIRS. It's not like seeing a close-up picture of Mars, which can tell you something about its geology right away. We have to look at the CIRS data from different times and sun angles to see how the ring temperatures are changing, then make computer models to test our theories on what those temperatures say about the rings."

The effort to understand the rings could help us understand our origin. "Our solar system formed from a dusty disk, so by understanding the dynamics in a disk like Saturn's rings, we can gain insight into how Earth and the other planets in our solar system were made," said Spilker.

The equators of both Earth and Saturn are tilted compared to their orbit around the sun. This tilt makes the sun appear to rise higher and lower in the sky throughout the year as Earth progresses in its orbit, causing the seasons to change. Likewise, Saturn's tilt makes the sun appear higher and lower in the sky as Saturn moves in its orbit, which takes about 29.5 years to complete.

Saturn experiences two equinoxes per orbit, just as Earth does, when the planet's equator lines up edge-on to its orbital plane, causing the sun to appear directly over the equator. For a viewer on Saturn, the sun would seem to move from south to north around the time of the August 11 equinox.

Technically, the equinox is the instant when the sun appears directly over the equator, but Saturn's situation gives the rings an extended twilight. Saturn is about 10 times farther from the sun than Earth. Since Saturn is farther from the Sun's gravitational pull, it moves relatively slowly in its orbit compared to Earth, which makes it take longer for the sun to noticeably appear higher or lower in the sky. Also, even as far away as Saturn, the sun is large enough to appear as a disk, not a point, according to Spilker.

So, before the August 11 equinox, a viewer embedded in Saturn's rings would have seen sunlight fade as the top edge of the solar disk appeared to touch the rings first. This would be followed by darkness around the equinox as the solar disk slowly crossed the ring plane. Full sunlight would have returned when the sun's bottom edge rose above the ring plane, about four days from when the sunlight first began to fade.

The Cassini-Huygens mission is a cooperative project among NASA and the European and Italian Space Agencies. NASA JPL manages the mission for the Science Mission Directorate at NASA Headquarters in Washington. JPL also designed, developed and assembled the Cassini orbiter and its two onboard cameras. The imaging team is based at the Space Science Institute, Boulder, Colo. The CIRS team is based at NASA Goddard. CIRS was built by Goddard, with significant hardware contributions from England and France.

Galaxy Cluster Smashes Distance Record

The most distant galaxy cluster yet has been discovered by combining data from NASA's Chandra X-ray Observatory and optical and infrared telescopes. The cluster is located about 10.2 billion light years away, and is observed as it was when the Universe was only about a quarter of its present age.

The galaxy cluster, known as JKCS041, beats the previous record holder by about a billion light years. Galaxy clusters are the largest gravitationally bound objects in the Universe. Finding such a large structure at this very early epoch can reveal important information about how the Universe evolved at this crucial stage.

JKCS041 is found at the cusp of when scientists think galaxy clusters can exist in the early Universe based on how long it should take for them to assemble. Therefore, studying its characteristics -- such as composition, mass, and temperature -- will reveal more about how the Universe took shape.

"This object is close to the distance limit expected for a galaxy cluster," said Stefano Andreon of the National Institute for Astrophysics (INAF) in Milan, Italy. "We don't think gravity can work fast enough to make galaxy clusters much earlier."

Distant galaxy clusters are often detected first with optical and infrared observations that reveal their component galaxies dominated by old, red stars. JKCS041 was originally detected in 2006 in a survey from the United Kingdom Infrared Telescope (UKIRT). The distance to the cluster was then determined from optical and infrared observations from UKIRT, the Canada-France-Hawaii telescope in Hawaii and NASA's Spitzer Space Telescope. Infrared observations are important because the optical light from the galaxies at large distances is shifted into infrared wavelengths because of the expansion of the universe.

The Chandra data were the final -- but crucial -- piece of evidence as they showed that JKCS041 was, indeed, a genuine galaxy cluster. The extended X-ray emission seen by Chandra shows that hot gas has been detected between the galaxies, as expected for a true galaxy cluster rather than one that has been caught in the act of forming.

Also, without the X-ray observations, the possibility remained that this object could have been a blend of different groups of galaxies along the line of sight, or a filament, a long stream of galaxies and gas, viewed front on. The mass and temperature of the hot gas detected estimated from the Chandra observations rule out both of those alternatives.

The extent and shape of the X-ray emission, along with the lack of a central radio source argue against the possibility that the X-ray emission is caused by scattering of cosmic microwave background light by particles emitting radio waves.

It is not yet possible, with the detection of just one extremely distant galaxy cluster, to test cosmological models, but searches are underway to find other galaxy clusters at extreme distances.

"This discovery is exciting because it is like finding a Tyrannosaurus Rex fossil that is much older than any other known," said co-author Ben Maughan, from the University of Bristol in the United Kingdom. "One fossil might just fit in with our understanding of dinosaurs, but if you found many more, you would have to start rethinking how dinosaurs evolved. The same is true for galaxy clusters and our understanding of cosmology."

The previous record holder for a galaxy cluster was 9.2 billion light years away, XMMXCS J2215.9-1738, discovered by ESA's XMM-Newton in 2006. This broke the previous distance record by only about 0.1 billion light years, while JKCS041 surpasses XMMXCS J2215.9 by about ten times that.

"What's exciting about this discovery is the astrophysics that can be done with detailed follow-up studies," said Andreon.

Among the questions scientists hope to address by further studying JKCS041 are: What is the build-up of elements (such as iron) like in such a young object? Are there signs that the cluster is still forming? Do the temperature and X-ray brightness of such a distant cluster relate to its mass in the same simple way as they do for nearby clusters?

The paper describing the results on JKCS041 from Andreon and his colleagues will appear in an upcoming issue of the journal Astronomy and Astrophysics. NASA's Marshall Space Flight Center in Huntsville, Ala., manages the Chandra program for NASA's Science Mission Directorate in Washington, DC. The Smithsonian Astrophysical Observatory controls Chandra's science and flight operations from Cambridge, Mass

Astronomers Find Organic Molecules Around Gas Planet

 Peering far beyond our solar system, NASA researchers have detected the basic chemistry for life in a second hot gas planet, advancing astronomers toward the goal of being able to characterize planets where life could exist. The planet is not habitable but it has the same chemistry that, if found around a rocky planet in the future, could indicate the presence of life.

"It's the second planet outside our solar system in which water, methane and carbon dioxide have been found, which are potentially important for biological processes in habitable planets," said researcher Mark Swain of NASA's Jet Propulsion Laboratory, Pasadena, Calif. "Detecting organic compounds in two exoplanets now raises the possibility that it will become commonplace to find planets with molecules that may be tied to life."

Swain and his co-investigators used data from two of NASA's orbiting Great Observatories, the Hubble Space Telescope and Spitzer Space Telescope, to study HD 209458b, a hot, gaseous giant planet bigger than Jupiter that orbits a sun-like star about 150 light years away in the constellation Pegasus. The new finding follows their breakthrough discovery in December 2008 of carbon dioxide around another hot, Jupiter-size planet, HD 189733b. Earlier Hubble and Spitzer observations of that planet had also revealed water vapor and methane.

The detections were made through spectroscopy, which splits light into its components to reveal the distinctive spectral signatures of different chemicals. Data from Hubble's near-infrared camera and multi-object spectrometer revealed the presence of the molecules, and data from Spitzer's photometer and infrared spectrometer measured their amounts.

"This demonstrates that we can detect the molecules that matter for life processes," said Swain. Astronomers can now begin comparing the two planetary atmospheres for differences and similarities. For example, the relative amounts of water and carbon dioxide in the two planets is similar, but HD 209458b shows a greater abundance of methane than HD 189733b. "The high methane abundance is telling us something," said Swain. "It could mean there was something special about the formation of this planet."

Other large, hot Jupiter-type planets can be characterized and compared using existing instruments, Swain said. This work will lay the groundwork for the type of analysis astronomers eventually will need to perform in shortlisting any promising rocky Earth-like planets where the signatures of organic chemicals might indicate the presence of life.

Rocky worlds are expected to be found by NASA's Kepler mission, which launched earlier this year, but astronomers believe we are a decade or so away from being able to detect any chemical signs of life on such a body.

If and when such Earth-like planets are found in the future, "the detection of organic compounds will not necessarily mean there's life on a planet, because there are other ways to generate such molecules," Swain said. "If we detect organic chemicals on a rocky, Earth-like planet, we will want to understand enough about the planet to rule out non-life processes that could have led to those chemicals being there."

"These objects are too far away to send probes to, so the only way we're ever going to learn anything about them is to point telescopes at them. Spectroscopy provides a powerful tool to determine their chemistry and dynamics."

You can follow the history of planet hunting from science fiction to science fact with NASA's PlanetQuest Historic Timeline at http://planetquest.jpl.nasa.gov/timeline/ .

This interactive web feature, developed by JPL, conveys the story of exoplanet exploration through a rich tapestry of words and images spanning thousands of years, beginning with the musings of ancient philosophers and continuing through the current era of space-based observations by NASA's Spitzer and Kepler missions. The timeline highlights milestones in culture, technology and science, and includes a planet counter that tracks the pace of exoplanet discoveries over time.

More information about exoplanets and NASA's planet-finding program is at http://planetquest.jpl.nasa.gov .

The Hubble Space Telescope is a project of international cooperation between NASA and the European Space Agency and is managed by NASA's Goddard Space Flight Center in Greenbelt, Md. The Space Telescope Science Institute, Baltimore, Md., conducts Hubble science operations. The institute is operated for NASA by the Association of Universities for research in Astronomy, Inc., Washington, D.C.

JPL manages the Spitzer Space Telescope mission for NASA. Science operations are conducted at the Spitzer Science Center at the California Institute of Technology in Pasadena. Caltech manages JPL for NASA.

Tool-making Human Ancestors Inhabited Grassland Environments Two Million Years Ago

In an article published in the open-access, peer-reviewed journal PLoS ONE on October 21, 2009, Dr Thomas Plummer of Queens College at the City University of New York, Dr Richard Potts of the Smithsonian Institution National Museum of Natural History and colleagues report the oldest archeological evidence of early human activities in a grassland environment, dating to 2 million years ago. The article highlights new research and its implications concerning the environments in which human ancestors evolved.

Scientists as far back as Charles Darwin have thought that adaptation to grassland environments profoundly influenced the course of human evolution. This idea has remained well-entrenched, even with recent recognition that hominin origins took place in a woodland environment and that the adaptive landscape in Africa fluctuated dramatically in response to short-term climatic shifts.

During the critical time period between 3 and 1.5 million years ago, the origin of lithic technology and archeological sites, the evolution of Homo and Paranthropus, selection for endurance running, and novel thermoregulatory adaptations to hot, dry environments in H. erectus have all been linked to increasingly open environments in Africa.

However, ecosystems in which grassland prevails have not been documented in the geological record of Pliocene hominin evolution, so it has been unclear whether open habitats were even available to hominins, and, if so, whether hominins utilized them. In their new study, Plummer and colleagues provide the first documentation of both at the 2-million-year-old Oldowan archeological site of Kanjera South, Kenya, which has yielded both Oldowan artifacts and well-preserved faunal remains, allowing researchers to reconstruct past ecosystems.

The researchers report chemical analyses of ancient soils and mammalian teeth, as well as other faunal data, from the ~2.0-million-year-old archeological sites at Kanjera South, located in western Kenya. The principal collaborating institutions of the Kanjera project are QueensCollege of the City University of New York, the Smithsonian Institution's Human Origins Program, and the NationalMuseums of Kenya. The findings demonstrate that the recently excavated archeological sites that preserve Oldowan tools, the oldest-known type of stone technology, were located in a grassland-dominated ecosystem during the crucial time period.

The study documents what was previously speculated based on indirect evidence -- that grassland-dominated ecosystems did, in fact, exist during the Plio-Pleistocene (ca. 2.5-1.5 million years ago) and that early human tool-makers were active in open settings. Other recent research shows that the Kanjera hominins obtained meat and bone marrow from a variety of animals and that they carried stone raw materials over surprisingly long distances in this grassland setting. A comparison with other Oldowan sites shows that by 2.0 million years ago, hominins, almost certainly of the genus Homo, lived in a wide range of habitats in East Africa, from open grassland to woodland and dry forest.

Plummer and colleagues conclude that early Homo was flexible in its habitat use and that the ability to find resources in both open and wooded habitats was a key part of its adaptation. This strongly contrasts with the habitat usage of older species of Australopithecus and appears to signify an important shift in early humans' use of the landscape.

Funding from the L. S. B. Leakey Foundation, the Leverhulme Trust, the National Geographic Society, the National Science Foundation, the Professional Staff Congress-City University of New York Research Award Program, and the Wenner-Gren Foundation for Kanjera field and laboratory research is gratefully acknowledged. Logistical support was provided by the Human Origins Program of the Smithsonian Institution. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.